RESEARCH SUPPORT

Research Support

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These services, which have been available since 1991, are managed by our Research Support Department and also supported by Prodia's Research & Esoteric Laboratory, Molecular Laboratory, and National Reference Center Laboratory. From 1991 to 2016, Prodia Clinical Laboratory has contributed to over 2.822 pieces of research for academic purposes, epidemiological studies, and scientific publications. These studies include more than 375 types of test comprising Research Use Only (RUO) tests, Laboratory Developing Tests (LDT), and molecular tests and over 100 types of routine tests. The research support department itself has a vision to become :

  1. A center of excellence for health and medical sciences
  2. A pioner for new research laboratory tests in Indonesia
  3. The best and biggest biomedical medicine research laboratory center in Indonesia

The research support services was established as a form of Prodia's commitment as a center of excellence to the development of sciences particularly in the field of clinical laboratory and generally in biomedical medicine in Indonesia.

The Scope of Research Support Services at Prodia Clinical Laboratory includes:

  1. Assisting in the search of information sources and literatures
  2. Facilitating research protocol establishment
  3. Providing information concerning tests related to research purposes, including routine tests, special tests, and new tests/research tests not yet routinely conducted in Indonesia
  4. Exploring the possibilities of conducting research tests
  5. Facilitating the procurement of research kit reagents (Research Use Only/RUO kits)
  6. Providing information on good and correct sample handling, method feasibility, and kit reagents to be used
  7. Assisting in handling, collecting, & storing research samples in -20°C or -70°C
  8. Performing research-related tests, including research tests, and making sure that test performances comply with procedures, methods, and equipment with reliable and trusted quality of test results
  9. Assisting in result interpretation
  10. Assisting in referencing of tests beyond its capacities
  11. Assisting in processing the research statistical data.

Prodia intends to establish collaborations with many faculties of medicine in Indonesia in the form of research and knowledge transfer, serving as a source of information on services and other aspects related to the research of:

  • The correct selection of biomarkers
  • Critical factors in pre-analytics, analytics, and post-analytics in a test
  • The most recent biomarker trends for research in a certain area, such as Cardiology, Gastroenterology, Pulmonology, Neurology, Ob-gyn, Hepatology, Pediatrics, Oncology, etc.
  • Methods & principles of tests (ELISA, molecular, GCMS, LCMS, and ICP-MS)
  • Research methodology
  • Manuscript preparation workshop
  • Etc

Prodia also seeks to participate in guest lectures as information providers related to the aspects of clinical laboratory management such as:

  • Quality control and stabilization
  • ISO accreditation process
  • Laboratory management
  • etc

It is also prepared to help with guest/external lecturers on medical, biomedical, and pathobiological laboratory for a disease :

  • Molecular biology (basic and advanced)
  • Stem cell
  • Disease pathobiology
  • etc.
What Is the Procedure for Conducting Research Tests at Prodia?

  1. The issuance of reagent prices within 3 workdays only applies to reagents for the ELISA method. Cost estimates for processing by GCMS, LC MSMS,ICP-MS, and molecular methods are issued within 10–20 workdays.
  2. For ELISA reagents, arrival time is between 6–8 weeks and for molecular, LC-MC, GC-MS reagents, 10–12 weeks.
  3. Test processing schedules are adjusted to time availability at the research laboratory. Please submit processing schedule applications 3 weeks before the plan.
  4. Please inform Prodia if you would like to follow the examination processing progress.
  5. The results issuance within 2–3 workdays only applies to the ELISA method. Results for GCMS, LC MSMS, ICP-MS, and molecular processing are issued within 7 workdays.
Research & Esoteric Laboratory and National Reference Center Laboratory

Some of the methods for research tests carried out in the research & esoteric laboratory and national reference center laboratory include:

  1. Chemiluminescent
  2. Enzyme Linked Fluorescent Assay (ELFA)
  3. Enzyme Linked Immuno Sorbent Assay (ELISA)
  4. Fluorescence Polarization Immunoassay (FPIA)
  5. Electrochemiluminescence Immunoassay (ECLIA)
  6. Immunoblotting
  7. Immunocromatography (Rapid Test)
  8. Coagulometry
  9. Colorimetry
  10. Microparticle Enzyme Immuno Assay (MEIA)
  11. Nephelometry
  12. Turbidimetry
  13. Flowcytometry
  14. High Performance Liquid Chromatography (HPLC) UV
  15. Liquid Chromatography Tandem Mass Spectrometry (LCMS)
  16. Gas Chromatography-Mass Spectrometry (GC-MS)
  17. Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)

Groups of research tests performed:

  • Anti-inflammatory cytokine
  • Pro-inflammatory cytokine
  • Plaque destabilization (MMP-1, MMP-2, MMP-9, MPO, ICAM-1, VCAM-1, P-selectin, E-selectin, etc.)
  • Plaque rupture (sCD40 Ligand, PlGF, PAPP-A)
  • Acute Phase reactant (sPLA2, Serum Amyloid A, Fibrinogen)
  • Cardiac ischemia (CK-MB, IMA, Myoglobin)
  • Cardiac necrosis (Troponin T, Troponin I, Fas Ligand)
  • Myocardial dysfunction (BNP, pro-BNP, ANP)
  • Angiogenesis (Endostatin, VEGF, VEGFR, Angiopoietin-1, Angiopoietin-2)
  • Oxidative stress (Isoprostane, MDA, MDA modified LDL, Small dense LDL, Ox-LDL)
  • Hypertension (ACE, ADMA, Aldosterone, Angiotensin II, NO, PRA)
  • Lipoprotein metabolism (Apo A II, CETP, FABP4, FABP5, LCAT, Pre ?1 HDL, sPLA2, LpPLA2, etc.)
  • Vascular calcification (OPG, RANKL, RANK, BMP-4, BMP-2, BMP-7, Fetuin-A, MGP, FGF23, etc.)
  • Stroke (MBP, NSE, Protein S-100B, MMP-9, GFAP)
  • Coagulation & bleeding (D-Dimer, Factor VII, Factor VIII, PAI-1, PAP-complex, Active Protein C, TAFI, t-PA, Thrombomodulin, vWF, Tissue Factor, etc.)
  • Diabetes (Adiponectin HMW & LMW, IAA, ICA, sRAGE, GLP-1, GLP-2, etc.)
  • Obesity (FFA, Ghrelin total, Leptin, Leptin receptor, Obestatin, Resistin, Visfatin, Vaspin, etc.)
  • Trace Element (Zn, Se, Mn, Cu, Fe, Co, Mg, Hg, Cd, Pb, As, Al, Li, Ni, Thallium, V)
  • Nutrition & Growth (Transferin, sTfR, Prealbumin, IGF-I, IGF-II, Vitamin A, E, C, EPO, Lactoferrin, etc.)
  • Preeclampsia (HPL, Endoglin, ADMA, sFlt-1, PlGF, dll)
  • Alzheimer's (? amyloid, Isoprostan, sPLA2, ApoE4)
  • Sepsis (LBP, PCT, Protein C)
  • Rheumatoid (Anti-CCP, COMP, MMP-3, TIMP-1, YKL-40)
  • dan others

Types of Specimen worked on include:

  • Blood
  • Urin
  • Feces
  • Sputum
  • Saliva
  • Throat swab
  • Vaginal swab
  • Body fluids/Brain/Joint/Lung
  • Tissues
  • etc
Molecular Laboratory

Prodia develops a number of molecular test methods that can be used for research, such as:

  • Conventional PCR with Electrophoresis gel detection
  • Allele-Specific PCR
  • PCR-Restriction Fragment Length Polymorphism
  • Real Time PCR (Fluoresence dye dan Probes)
  • Nucleic Acid Sequence Based Amplification (NASBA)
  • Line-Probe Assay

Facilities at the Molecular Laboratory :

  • Biosafety Cabinet for preparing samples and reagents
  • Cobas AmpliPrep/Cobas Taqman (Automatic Extraction/Real Time PCR)
  • Thermal Cycler GeneAmp 2400, Veriti (Conventional PCR)
  • Electrophoresis Gel
  • Gel Documentation (GelDoc)
  • Light Cycler 2.0 (Real Time PCR)
  • Nanodrop (For DNA/RNA/Protein quantifications)
  • R2-M Flow-through System (Flow through hybridization)
  • Cobas 4800 ( Chlamydia trachomatis-Neisseria gonorhae DNA dengan PCR
  • GeneXpert (BCR-ABL Kuantitatif)
  • Rotorgene (Real Time PCR)
  • Veriti (Konvensional PCR)
  • Sequencing
  • Applied Biosystem Genetic Analyzer  3500

Available tools :

  • Biosafety Cabinet for preparing samples and reagents
  • Cobas AmpliPrep/Cobas Taqman (Automatic Extraction/Real Time PCR)
  • Thermal Cycler GeneAmp 2400
  • Electrophoresis Gel
  • Gel Documentation (GelDoc)
  • Light Cycler 2.0 (Real Time PCR)
  • Nanodrop (For DNA/RNA/Protein quantifications)
  • Nuclisens EasyMag (Automatic DNA/RNA extraction) by the Boom method
  • Nuclisens EasyQ (NASBA)
  • Shaker Waterbath for Line Probe method processing

Types of performable test :
Detection and Quantification of Pathogens (virus, bacteria, parasites, etc)

  • Serotyping dengue
  • Toxoplasmosis
  • Chlamydia trachomatis
  • Neisseria gonorhoeae
  • Circulating DNA
  • Detection of cccDNA
  • and others
Gen Expression (relative/absolute quantification)
  • VEGFR
  • and others
Types of Test
For types of performable test, please see here!
Research Collaborations

To expand its networks as well as to improve the quality of research and to obtain information on the latest development in medical sciences, Prodia Clinical Laboratory cooperates in research, education, and community services with faculties of medicine at many universities in Indonesia, including:

  1. Faculty of Medicine at Sumatera Utara University (USU), Medan
  2. Faculty of Medicine at Hasanuddin University (UNHAS), Makassar
  3. Faculty of Medicine at Sam Ratulangi University (UNSRAT), Manado
  4. Faculty of Medicine at Mulawarman University (UNMUL), Samarinda
  5. Faculty of Medicine at Maranatha Christian University, Bandung
  6. Faculty of Medicine at Syiah Kuala University, Banda Aceh
  7. Faculty of Medicine at Udayana University, Denpasar
  8. Faculty of Medicine at Jenderal Soedirman University (UNSOED), Purwokerto
  9. Faculty of Medicine at Haluoleo University, Kendari
  10. Faculty of Medicine at Gadjah Mada University (UGM), Yogyakarta
  11. Faculty of Medicine at Batam University (UNIBA), Batam
  12. Faculty of Medicine at Riau University (UR), Pekanbaru
  13. Faculty of Medicine at Sebelas Maret University, Solo
  14. Faculty of Medicine at Al-Khairat University, Palu
  15. Faculty of Medicine at Diponegoro University (UNDIP), Semarang
  16. Faculty of Medicine at Jenderal Achmad Yani University (Unjani), Bandung
  17. Faculty of Medicine at Sriwijaya University (UNSRI), Palembang
  18. Faculty of Medicine at Indonesia Moslem University (UMI), Makassar
  19. Faculty of Medicine at Andalas University (UNAND), Padang
  20. Faculty of Medicine at Bandung Islamic University (UNISBA), Bandung
  21. Faculty of Pharmacy at Padjajaran University
  22. Faculty of Medicine at Trisakti University
  23. University of Indonesia
  24. Faculty of Medicine at Brawijaya University (UB), Surabaya
  25. Faculty of Medicine at Lambung Mangkurat University (ULM), Banjarmasin
  26. Faculty of Medicine at Tanjungpura University, Pontianak
Research 1991 - 2016

Image : during 24 years in the field of medical especially science research Prodia has helped 677 Doctoral Research and 1121 research for the purpose of specialization, etc.

 

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